UVProbe Basic Procedures for Spectrum Collection
1. Turn ON power for PC, Monitor and Spectrophotometer
2. Starting the Software
Double click UVProbe icon on desktop to launch UVProbe software.
This screen should appear once the software is started. The software is divided into 4 modules: Spectrum, Photometrics (Quantitation), Kinetics, and Report Generator. These Modules can be selected by clicking on the correct Icon or selecting the module from the Window pull down menu.
3. Spectrum Window
These various windows can be customized for the users preference by turning them on or off in the View Menu.
4. Connect the Instrument
A. Select [Window] > Spectrum to open the Spectrum module. A module must be active before the spectrophotometer can be connected.
B. Click [Connect]. A dialog box will appear showing the progress of the connection.
5. Creating a Method: Setting Measurement Parameters
Select Edit > Method, or click the Method icon to display the Spectrum Method dialog box. The Method parameters are arranged in a tab format. The measurement tab allows you to set up the parameters for your scan. If you are going to run a sample, input the Start and End wavelength values. The Sample Preparation tab can be used to record information about the sample, but does not affect the measurement.
The Instrument Parameters tab is used to determine the instrument settings. The important settings here are the measuring mode and the Slit Width. Set these for your sample.
6. Data Collection
Confirm that no cuvettes are in sample and reference beams.
Click [Baseline] on the Photometer Button bar at the bottom of the screen and click [Ok] in the Baseline Parameters dialog box. Remember, Baseline corrects over the entire selected wavelength range. The Autozero function corrects at only one wavelength.
Perform a spectral scan by inserting the sample and reference into the spectrophotometer. Click the [Start] button. After the scan, enter a file name in the New Data Set dialog box and click [Ok].
7. Data Saving
Select File > [Save As] and select the appropriate data directory in the [Save In] box at the top of the dialog box. Enter the correct file name, select .spc in the [Save As Type] list, and click [Save].
8. Data Display
If data has not been collected, open sample data. The Graph pane on the right of the screen has 3 tabs. The Active tab shows one single spectrum. This is the data that will be manipulated.
Note: To delete displayed data, click [Files], [Properties], highlight selected data, then click [Delete]
To save displayed data, click [Files], [Properties], highlight selected data, then click [Save]
The Overlay tab will show all open spectral data on the same graph. This allows easy comparison between samples. During data collection, the spectra will appear in the Overlay tab.
The Stacked tab displays all open spectral data in individual graphs. Spectra can be chosen to be displayed or not by using the legend function.
UV Probe has vast data processing capabilities. Most calculations can be done under the Manipulation menu. The Manipulation pane is in the top left corner of the screen. Calculation results and information will show up in this pane.
A. Data Print: Shows a table of the raw data of Absorbance or Transmittance versus the wavelength for the open spectra. This table can be copied and pasted into other programs such as Excel
B. Manipulate: This gives a variety of mathematical operations that can be done on one or more data sets.
C. Peak Pick: Results in a Peak table for the open spectral data. Descriptions can be added to the peaks or valleys, and the results can be annotated on the graph.
D. Point Pick: Gives a table of results for specified wavelengths in a spectra. These wavelengths can be saved as a template for repeat use on multiple spectra.
E. PICKING: Right click on the active pane, grab vertical line and drag to desired point, click wavelength selected, then click on next table line for next point input
F. Peak Area: Provides area under peak results for wavelength regions within the spectra. These areas are indicated by shading on the graph.
The flexible Report Generator function allows the use of custom reports with a variety of collected data and information included.
A. Insert Typed Text: Refers to text that may be input by the user directly onto the report. This text may include the Report Title, Company Name, or any other information that will not change with each report.
1. Go to [Insert]>Text. A Text Box will appear in the Report Window, type in desired text.
2. Size of the Text box can be changed by clicking and dragging corners of boxes into desired shape. The Font Properties may be changed by selecting [View]>Properties.
B. Insert Linked Text: Refers to Text that may change with each report and will be automatically inserted by the software. This text may include Creation Date, Logged in User, or File Name.
1. Go to [Insert]>Field. A Linked Text Properties box will appear with Field Names and Categories to choose from.
2. Select the Desired Category (i.e. Spectrum) to get a list of available Field Names
3. Double click on the desired Field Name and exit out of the Properties Box.
4. The linked information will appear in the Report Window. The size and position of the Text Box can be changed.
C. Insert Linked Data: Refers to data found in other open modules of UV Probe. This link may be created between the report and the Active Spectrum, Standard Table, Enzyme Table, or any other data that is associated with a file. The report data may change when new files are opened in other modules.
1. Open the Data file in the appropriate module. Go to [Insert]>[Insert Object]>Appropriate Module (i.e. Spectrum).
2. Select the desired Data Type (i.e. Graph)
3. A Data Box will appear in the Report Window. Right click on the Data Box and select Properties.
4. A Properties box will appear with listings of data. Put a check mark by the desired data for the link.
5. To see whether the data you requested has been linked, go to [File]>[Print Preview]. You should see a setup of your page with the linked data requested.
